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KMID : 0353320020260010149
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Oral Biology Research
2002 Volume.26 No. 1 p.149 ~ p.159
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Effects of transfection reagents Superfect and Tfx on the RANKL-induced differentiation of RAW cells into osteoclast-like cells
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Abstract
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The ability to introduce DNA into mammalian cells provides a powerful means to study the function and control of mammalian genes. Common techniques for gene (DNA) transfer into eukaryotic cells include calcium phosphate. DEAE-Dextran, electroporation and more recently, liposome-mediated transfection. When reagents for liposome-mediated transfection were tested with RAW264.7 cells that can differentiate into osteoclast-like cells upon RANKL treatment. Superfect was found to reduce the differentiation whereas TfxTM had no effects. Therefore, TfxTM may be a more suitable reagent fot introducing DNA into cells in osteoclastogenesis studies.
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